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Identification of rare levels of methylated tumor DNA fragments using an optimized bias based pre-amplification-digital droplet PCR (OBBPA-ddPCR)

BACKGROUND: The analysis of aberrant DNA methylations is used for the diagnosis of cancer as significant changes in the gene methylation pattern are often detected during early carcinogenesis. In this study, we evaluated the performance of a two-step method that combines pre-amplification with ddPCR...

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Publicat a:Oncotarget
Autors principals: Menschikowski, Mario, Jandeck, Carsten, Friedemann, Markus, Nacke, Brit, Hantsche, Saskia, Tiebel, Oliver, Sukocheva, Olga, Hagelgans, Albert
Format: Artigo
Idioma:Inglês
Publicat: Impact Journals LLC 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6281424/
https://ncbi.nlm.nih.gov/pubmed/30546833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.18632/oncotarget.26315
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