A simple method to increase the proportion of bone marrow-derived macrophages positive for M-CSFR using the reducing agent dithiothreitol (DTT)

Only a few bone marrow-derived macrophages (BM-MΦ) are positive for macrophage colony-stimulating factor receptor (M-CSFR). Thus, a method is needed to increase the proportion of BM-MΦ that are positive for M-CSFR to facilitate the investigation of the effects of M-CSFR downregulation on various dis...

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Detalhes bibliográficos
Publicado no:MethodsX
Main Authors: Hashimoto, Ryota, Daida, Hiroyuki, Okada, Takao, Katoh, Youichi
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2018
Assuntos:
Biochemistry, Genetics and Molecular Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6279884/
https://ncbi.nlm.nih.gov/pubmed/30555796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mex.2018.11.014
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