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Large-scale reconstruction of cell lineages using single-cell readout of transcriptomes and CRISPRCas9 barcodes by scGESTALT

Lineage relationships among the large number of heterogeneous cell types generated during development are difficult to reconstruct in a high-throughput manner. We recently established a method, scGESTALT, that combines cumulative editing of a lineage barcode array by CRISPR-Cas9 with large-scale tra...

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Publicat a:Nat Protoc
Autors principals: Raj, Bushra, Gagnon, James A., Schier, Alexander F.
Format: Artigo
Idioma:Inglês
Publicat: 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6279253/
https://ncbi.nlm.nih.gov/pubmed/30353175
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41596-018-0058-x
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