Exocyst dynamics during vesicle tethering and fusion

The exocyst is a conserved octameric complex that tethers exocytic vesicles to the plasma membrane prior to fusion. Exocyst assembly and delivery mechanisms remain unclear, especially in mammalian cells. Here we tagged multiple endogenous exocyst subunits with sfGFP or Halo using Cas9 gene-editing,...

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Udgivet i:Nat Commun
Main Authors: Ahmed, Syed Mukhtar, Nishida-Fukuda, Hisayo, Li, Yuchong, McDonald, W. Hayes, Gradinaru, Claudiu C., Macara, Ian G.
Format: Artigo
Sprog:Inglês
Udgivet: Nature Publishing Group UK 2018
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Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6277416/
https://ncbi.nlm.nih.gov/pubmed/30510181
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-07467-5
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