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Site-Selective Ribosylation of Fluorescent Nucleobase Analogs Using Purine-Nucleoside Phosphorylase as a Catalyst: Effects of Point Mutations

Enzymatic ribosylation of fluorescent 8-azapurine derivatives, like 8-azaguanine and 2,6-diamino-8-azapurine, with purine-nucleoside phosphorylase (PNP) as a catalyst, leads to N9, N8, and N7-ribosides. The final proportion of the products may be modulated by point mutations in the enzyme active sit...

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Bibliografiske detaljer
Udgivet i:Molecules
Main Authors: Stachelska-Wierzchowska, Alicja, Wierzchowski, Jacek, Bzowska, Agnieszka, Wielgus-Kutrowska, Beata
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2015
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6274182/
https://ncbi.nlm.nih.gov/pubmed/26729076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules21010044
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