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A sensitive and improved throughput UPLC-MS/MS quantitation method of total cytochrome P450 mediated arachidonic acid metabolites that can separate regio-isomers and cis/trans-EETs from human plasma

A method for the detection and quantification of hydroxyl and epoxy arachidonic acid (AA(1)) metabolites in human plasma was developed using liquid-liquid extraction, phospholipid saponification followed by derivatization of the acid moiety and liquid chromatographic tandem mass spectrometric detect...

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Detalhes bibliográficos
Publicado no:Chem Phys Lipids
Main Authors: Zeigler, Maxwell, Whittington, Dale, Sotoodehnia, Nona, Lemaitre, Rozenn N., Totah, Rheem A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6269592/
https://ncbi.nlm.nih.gov/pubmed/30201384
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chemphyslip.2018.09.004
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