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In utero electroporation-based translating ribosome affinity purification identifies age-dependent mRNA expression in cortical pyramidal neurons

We combined translating ribosome affinity purification (TRAP) with in utero electroporation (IUE), called iTRAP to identify the molecular profile of specific neuronal populations during neonatal development without the need for viral approaches and FACS sorting. We electroporated a plasmid encoding...

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Bibliographische Detailangaben
Veröffentlicht in:Neurosci Res
Hauptverfasser: Huang, Tianxiang, Nguyen, Lena, Lin, Tiffany V., Gong, Xuan, Zhang, Longbo, Kim, Gi Bum, Sarkisian, Matthew R., Breunig, Joshua, Bordey, Angelique
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6265126/
https://ncbi.nlm.nih.gov/pubmed/29857015
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.neures.2018.05.006
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