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Optimal molecular crowding accelerates group II intron folding and maximizes catalysis
Unlike in vivo conditions, group II intron ribozymes are known to require high magnesium(II) concentrations ([Mg(2+)]) and high temperatures (42 °C) for folding and catalysis in vitro. A possible explanation for this difference is the highly crowded cellular environment, which can be mimicked in vit...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6255197/ https://ncbi.nlm.nih.gov/pubmed/30397128 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1806685115 |
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