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Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Global defects in RNA polymerase II transcription might be overlooked by transcriptomic studies analyzing steady-state RNA. Indeed, the global decrease in mRNA synthesis has been shown to be compensated by a simultaneous decrease in mRNA degradation to restore normal steady-state levels. Hence, the...

詳細記述

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書誌詳細
出版年:J Vis Exp
主要な著者: Baptista, Tiago, Devys, Didier
フォーマット: Artigo
言語:Inglês
出版事項: MyJove Corporation 2018
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235584/
https://ncbi.nlm.nih.gov/pubmed/30394386
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57982
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