Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Folding reporters are proteins with easily identifiable phenotypes, such as antibiotic resistance, whose folding and function is compromised when fused to poorly folding proteins or random open reading frames. We have developed a strategy where, by using TEM-1 β-lactamase (the enzyme conferring ampi...

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Vydáno v:J Vis Exp
Hlavní autoři: Soluri, Maria Felicia, Puccio, Simone, Caredda, Giada, Grillo, Giorgio, Licciulli, Vito Flavio, Consiglio, Arianna, Edomi, Paolo, Santoro, Claudio, Sblattero, Daniele, Peano, Clelia
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2018
Témata:
Biology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235410/
https://ncbi.nlm.nih.gov/pubmed/30346377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56981
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