Interactome-Seq: A Protocol for Domainome Library Construction, Validation and Selection by Phage Display and Next Generation Sequencing

Folding reporters are proteins with easily identifiable phenotypes, such as antibiotic resistance, whose folding and function is compromised when fused to poorly folding proteins or random open reading frames. We have developed a strategy where, by using TEM-1 β-lactamase (the enzyme conferring ampi...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Soluri, Maria Felicia, Puccio, Simone, Caredda, Giada, Grillo, Giorgio, Licciulli, Vito Flavio, Consiglio, Arianna, Edomi, Paolo, Santoro, Claudio, Sblattero, Daniele, Peano, Clelia
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2018
Schlagworte:
Biology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235410/
https://ncbi.nlm.nih.gov/pubmed/30346377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/56981
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