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Generation of High-Throughput Three-Dimensional Tumor Spheroids for Drug Screening

Cancer cells have routinely been cultured in two dimensions (2D) on a plastic surface. This technique, however, lacks the true environment a tumor mass is exposed to in vivo. Solid tumors grow not as a sheet attached to plastic, but instead as a collection of clonal cells in a three-dimensional (3D)...

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Griner, Lesley Mathews, Gampa, Kalyani, Do, Toan, Nguyen, Huyen, Farley, David, Hogan, Christopher J., Auld, Douglas S., Silver, Serena J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2018
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6235107/
https://ncbi.nlm.nih.gov/pubmed/30247463
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57476
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