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Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion

Studies in dynamic changes in protein translation require specialized methods. Here we examined changes in newly-synthesized proteins in response to ischemia and reperfusion using the isolated perfused mouse heart coupled with polysome profiling. To further understand the dynamic changes in protein...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Stastna, Miroslava, Thomas, Amandine, Germano, Juliana, Pourpirali, Somayeh, Van Eyk, Jennifer E., Gottlieb, Roberta A.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6231915/
https://ncbi.nlm.nih.gov/pubmed/30222143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58079
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