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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

Primary human keratinocytes isolated from fresh skin tissues and their expansion in vitro have been widely used for laboratory research and for clinical applications. The conventional isolation method of human keratinocytes involves a two-step sequential enzymatic digestion procedure, which has been...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Liu, Zhenan, Wen, Jie, Leng, Xue, Zhou, Qian, Zhou, Changkuo, Zhao, Huaqiang, Wu, Xunwei
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6231857/
https://ncbi.nlm.nih.gov/pubmed/30199027
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57784
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