Strategic single point mutation yields a solvent- and salt-stable transaminase from Virgibacillus sp. in soluble form

A new transaminase (VbTA) was identified from the genome of the halotolerant marine bacterium Virgibacillus 21D. Following heterologous expression in Escherichia coli, it was located entirely in the insoluble fraction. After a single mutation, identified via sequence homology analyses, the VbTA T16F...

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Publicado no:Sci Rep
Main Authors: Guidi, Benedetta, Planchestainer, Matteo, Contente, Martina Letizia, Laurenzi, Tommaso, Eberini, Ivano, Gourlay, Louise J., Romano, Diego, Paradisi, Francesca, Molinari, Francesco
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2018
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6219536/
https://ncbi.nlm.nih.gov/pubmed/30401905
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-34434-3
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