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Crystallization of ectonucleotide phosphodiesterase/pyrophosphatase-3 and orientation of the SMB domains in the full-length ectodomain

Ectonucleotide phosphodiesterase/pyrophosphatase-3 (NPP3, ENPP3) is an ATP-hydrolyzing glycoprotein that is located in the extracellular space. The full-length ectodomain of rat NPP3 was expressed in HEK293S GntI(−) cells, purified using two chromatographic steps and crystallized. Its structure at 2...

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Bibliografische gegevens
Gepubliceerd in:Acta Crystallogr F Struct Biol Commun
Hoofdauteurs: Döhler, Christoph, Zebisch, Matthias, Krinke, Dana, Robitzki, Andrea, Sträter, Norbert
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: International Union of Crystallography 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6213977/
https://ncbi.nlm.nih.gov/pubmed/30387774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X18011111
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