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Screening of candidate substrates and coupling ions of transporters by thermostability shift assays

Substrates of most transport proteins have not been identified, limiting our understanding of their role in physiology and disease. Traditional identification methods use transport assays with radioactive compounds, but they are technically challenging and many compounds are unavailable in radioacti...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Majd, Homa, King, Martin S, Palmer, Shane M, Smith, Anthony C, Elbourne, Liam DH, Paulsen, Ian T, Sharples, David, Henderson, Peter JF, Kunji, Edmund RS
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6211832/
https://ncbi.nlm.nih.gov/pubmed/30320551
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.38821
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