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Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O‑GlcNAc Stoichiometry by Western Blotting
The dynamic modification of intracellular proteins by O-linked β-N-acetylglucosamine (O-GlcNAcylation) plays critical roles in many cellular processes. Although various methods have been developed for O-GlcNAc detection, there are few techniques for monitoring glycosylation stoichiometry and state (...
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| Veröffentlicht in: | Biochemistry |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2018
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6211186/ https://ncbi.nlm.nih.gov/pubmed/30169966 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.biochem.8b00648 |
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