A protocol combining multiphoton microscopy and propidium iodide for deep 3D root meristem imaging in rice: application for the screening and identification of tissue-specific enhancer trap lines

BACKGROUND: The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and in vivo growth monitoring. Confocal microscopy coupled with the use of propidium...

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Detalles Bibliográficos
Publicado en:Plant Methods
Main Authors: Bureau, Charlotte, Lanau, Nadège, Ingouff, Mathieu, Hassan, Boukhaddaoui, Meunier, Anne-Cécile, Divol, Fanchon, Sevilla, Rosie, Mieulet, Delphine, Dievart, Anne, Périn, Christophe
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2018
Assuntos:
Methodology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC6206838/
https://ncbi.nlm.nih.gov/pubmed/30386414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-018-0364-x
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