CRISPR-Cas9 Mediated Epitope Tagging Provides Accurate and Versatile Assessment of Myocardin

OBJECTIVE –: Unreliable antibodies often hinder the accurate detection of an endogenous protein, and this is particularly true for the cardiac and smooth muscle cofactor, Myocardin (MYOCD). Accordingly, the mouse Myocd locus was targeted with two independent epitope tags for the unambiguous expressi...

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Veröffentlicht in:Arterioscler Thromb Vasc Biol
Hauptverfasser: Lyu, Qing, Dhagia, Vidhi, Han, Yu, Guo, Bing, Wines-Samuelson, Mary E., Christie, Christine K., Yin, Qiangzong, Slivano, Orazio J., Herring, Paul, Long, Xiaochun, Gupte, Sachin A., Miano, Joseph M.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2018
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Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6204210/
https://ncbi.nlm.nih.gov/pubmed/29976770
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1161/ATVBAHA.118.311171
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