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Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region

BACKGROUND: High thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein thermostability. RESULTS: The flexible regions in porcine trypsin were predicted...

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Podrobná bibliografie
Vydáno v:Microb Cell Fact
Hlavní autoři: Liu, Lin, Yu, Haoran, Du, Kun, Wang, Zhiyan, Gan, Yiru, Huang, He
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2018
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6201580/
https://ncbi.nlm.nih.gov/pubmed/30359279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-018-1012-x
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