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Enhanced trypsin thermostability in Pichia pastoris through truncating the flexible region
BACKGROUND: High thermostability is required for trypsin to have wider industrial applications. Target mutagenesis at flexible regions has been proved to be an efficient protein engineering method to enhance the protein thermostability. RESULTS: The flexible regions in porcine trypsin were predicted...
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| Publicado no: | Microb Cell Fact |
|---|---|
| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6201580/ https://ncbi.nlm.nih.gov/pubmed/30359279 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-018-1012-x |
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