Dynamic coordination of two-metal-ions orchestrates λ-exonuclease catalysis

Metal ions at the active site of an enzyme act as cofactors, and their dynamic fluctuations can potentially influence enzyme activity. Here, we use λ-exonuclease as a model enzyme with two Mg(2+) binding sites and probe activity at various concentrations of magnesium by single-molecule-FRET. We find...

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Pubblicato in:Nat Commun
Autori principali: Hwang, Wonseok, Yoo, Jungmin, Lee, Yuno, Park, Suyeon, Hoang, Phuong Lien, Cho, HyeokJin, Yu, Jeongmin, Hoa Vo, Thi Minh, Shin, Minsang, Jin, Mi Sun, Park, Daeho, Hyeon, Changbong, Lee, Gwangrog
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group UK 2018
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC6199318/
https://ncbi.nlm.nih.gov/pubmed/30353000
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-018-06750-9
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