A protein–protein host–guest complex: Thermostable ferritin encapsulating positively supercharged green fluorescent protein

We characterize the encapsulation of supercharged green fluorescent protein, GFP(+36), by thermophilic ferritin from Archaeoglobus fulgidus (AfFtn). The AfFtn–GFP(+36) assembly is rapid, nearly stoichiometric, and robust. Using a more stably assembled mutant AfFtn, we show that encapsulation can occ...

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Publicat a:Protein Sci
Autors principals: Pulsipher, Katherine W., Bulos, Joshua A., Villegas, José A., Saven, Jeffery G., Dmochowski, Ivan J.
Format: Artigo
Idioma:Inglês
Publicat: John Wiley & Sons, Inc. 2018
Matèries:
Full‐Length Papers
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6199156/
https://ncbi.nlm.nih.gov/pubmed/30051936
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.3483
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