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Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments

CRISPR (clustered regularly interspaced short palindromic repeats) genome-editing experiments offer enormous potential for the evaluation of genomic loci using arrayed single guide RNARNARNAs (sgRNAs) or pooled sgRNA libraries. Numerous computational tools are available to help design sgRNAs with op...

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Detalhes bibliográficos
Publicado no:Nat Protoc
Main Authors: Canver, Matthew C, Haeussler, Maximilian, Bauer, Daniel E, Orkin, Stuart H, Sanjana, Neville E, Shalem, Ophir, Yuan, Guo-Cheng, Zhang, Feng, Concordet, Jean-Paul, Pinello, Luca
Formato: Artigo
Idioma:Inglês
Publicado em: 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6182299/
https://ncbi.nlm.nih.gov/pubmed/29651054
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.005
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