Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments

CRISPR (clustered regularly interspaced short palindromic repeats) genome-editing experiments offer enormous potential for the evaluation of genomic loci using arrayed single guide RNARNARNAs (sgRNAs) or pooled sgRNA libraries. Numerous computational tools are available to help design sgRNAs with op...

Celý popis

Uloženo v:
Podrobná bibliografie
Vydáno v:Nat Protoc
Hlavní autoři: Canver, Matthew C, Haeussler, Maximilian, Bauer, Daniel E, Orkin, Stuart H, Sanjana, Neville E, Shalem, Ophir, Yuan, Guo-Cheng, Zhang, Feng, Concordet, Jean-Paul, Pinello, Luca
Médium: Artigo
Jazyk:Inglês
Vydáno: 2018
Témata:
Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC6182299/
https://ncbi.nlm.nih.gov/pubmed/29651054
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2018.005
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky