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Yeast Ded1 promotes 48S translation pre-initiation complex assembly in an mRNA-specific and eIF4F-dependent manner

DEAD-box RNA helicase Ded1 is thought to resolve secondary structures in mRNA 5'-untranslated regions (5'-UTRs) that impede 48S preinitiation complex (PIC) formation at the initiation codon. We reconstituted Ded1 acceleration of 48S PIC assembly on native mRNAs in a pure system, and recapi...

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Bibliografische gegevens
Gepubliceerd in:eLife
Hoofdauteurs: Gupta, Neha, Lorsch, Jon R, Hinnebusch, Alan G
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: eLife Sciences Publications, Ltd 2018
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6181565/
https://ncbi.nlm.nih.gov/pubmed/30281017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.38892
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