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Enhancement of PCR Sensitivity and Yield Using Thiol-modified Primers
Various additives can enhance the quality of PCR amplification, but these generally require considerable optimization to achieve peak performance. Here, we demonstrate that the use of thiol-modified primers can enhance both PCR sensitivity and yield. In experiments with V. parahaemolyticus genomic D...
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| Pubblicato in: | Sci Rep |
|---|---|
| Autori principali: | , , , , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Nature Publishing Group UK
2018
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6173752/ https://ncbi.nlm.nih.gov/pubmed/30291287 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-33223-2 |
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