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Microtubule organization within mitotic spindles revealed by serial block face scanning electron microscopy and image analysis
Serial block face scanning electron microscopy (SBF-SEM) is a powerful method to analyze cells in 3D. Here, working at the resolution limit of the method, we describe a correlative light–SBF-SEM workflow to resolve microtubules of the mitotic spindle in human cells. We present four examples of uses...
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| Publicado no: | J Cell Sci |
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| Principais autores: | , , , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2017
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6173286/ https://ncbi.nlm.nih.gov/pubmed/28389579 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/jcs.203877 |
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