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Crystal structures of green fluorescent protein with the unnatural amino acid 4-nitro-l-phenylalanine

The X-ray crystal structures of two superfolder green fluorescent protein (sfGFP) constructs containing a genetically incorporated spectroscopic reporter unnatural amino acid, 4-nitro-l-phenylalanine (pNO(2)F), at two unique sites in the protein have been determined. Amber codon-suppression methodol...

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Detalhes bibliográficos
Publicado no:Acta Crystallogr F Struct Biol Commun
Main Authors: Maurici, Nicole, Savidge, Nicole, Lee, Byung Uk, Brewer, Scott H., Phillips-Piro, Christine M.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2018
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6168768/
https://ncbi.nlm.nih.gov/pubmed/30279317
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X1801169X
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