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Improving yields of deuterated, methyl labeled protein by growing in H(2)O
Solution NMR continues to make strides in addressing protein systems of significant size and complexity. A fundamental requirement to fully exploit the (15)N-(1)H TROSY and (13)C-(1)H(3) methyl TROSY effects is highly deuterated protein. Unfortunately, traditional overexpression E. coli during growt...
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| Izdano u: | J Biomol NMR |
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| Glavni autori: | , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2018
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6165672/ https://ncbi.nlm.nih.gov/pubmed/30073492 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-018-0200-7 |
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