Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion

This protocol describes reconstitution assays to study how the neurotransmitter release machinery triggers Ca(2+)-dependent synaptic vesicle fusion. The assays monitor fusion between proteoliposomes containing the synaptic vesicle SNARE synaptobrevin (with or without the Ca(2+) sensor synaptotagmin-...

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Bibliographic Details
Published in:Nat Protoc
Main Authors: Liu, Xiaoxia, Seven, Alpay Burak, Xu, Junjie, Esser, Victoria, Su, Lijing, Ma, Cong, Rizo, Josep
Format: Artigo
Language:Inglês
Published: 2017
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6163043/
https://ncbi.nlm.nih.gov/pubmed/28858288
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.068
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