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Simultaneous lipid and content mixing assays for in vitro reconstitution studies of synaptic vesicle fusion
This protocol describes reconstitution assays to study how the neurotransmitter release machinery triggers Ca(2+)-dependent synaptic vesicle fusion. The assays monitor fusion between proteoliposomes containing the synaptic vesicle SNARE synaptobrevin (with or without the Ca(2+) sensor synaptotagmin-...
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| Publicat a: | Nat Protoc |
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| Autors principals: | , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2017
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6163043/ https://ncbi.nlm.nih.gov/pubmed/28858288 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.068 |
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