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Microscale reversed-phase liquid chromatography-capillary zone electrophoresis-tandem mass spectrometry for deep and highly sensitive bottom-up proteomics: identification of 7500 proteins with five micrograms of an MCF7 proteome digest

Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been well recognized for bottom-up proteomics. It has approached 4000-8000 protein identifications (IDs) from a human cell line, mouse brains or Xenopus embryos via coupling with liquid chromatography (LC) prefractionation. Howe...

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Publicat a:Anal Chem
Autors principals: Yang, Zhichang, Shen, Xiaojing, Chen, Daoyang, Sun, Liangliang
Format: Artigo
Idioma:Inglês
Publicat: 2018
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6156779/
https://ncbi.nlm.nih.gov/pubmed/30102516
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b02466
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