Single-Homology-Arm Linear DNA Recombination by the Nonhomologous End Joining Pathway as a Novel and Simple Gene Inactivation Method: a Proof-of-Concept Study in Dietzia sp. Strain DQ12-45-1b

Nonhomologous end joining (NHEJ) is critical for genome stability because of its roles in double-strand break repair. Ku and ligase D (LigD) are the crucial proteins in this process, and strains expressing Ku and LigD can cyclize linear DNA in vivo. Here, we established a proof-of-concept single-hom...

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Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Lu, Shelian, Nie, Yong, Wang, Meng, Xu, Hong-Xiu, Ma, Dong-Ling, Liang, Jie-Liang, Wu, Xiao-Lei
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2018
Schlagworte:
Biotechnology
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6146981/
https://ncbi.nlm.nih.gov/pubmed/30030230
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00795-18
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