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Colonoscopy-based colorectal cancer modeling in mice with CRISPR-Cas9 genome editing and organoid transplantation
Most genetically engineered mouse models of colorectal cancer are limited by tumor formation in the small intestine, a high tumor burden that limits metastasis, and the need to generate and cross mutant mice. Cell line or organoid transplantation models generally produce tumors in ectopic locations,...
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| Publicado no: | Nat Protoc |
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| Main Authors: | , , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6145089/ https://ncbi.nlm.nih.gov/pubmed/29300388 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.136 |
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