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Colonoscopy-based colorectal cancer modeling in mice with CRISPR-Cas9 genome editing and organoid transplantation

Most genetically engineered mouse models of colorectal cancer are limited by tumor formation in the small intestine, a high tumor burden that limits metastasis, and the need to generate and cross mutant mice. Cell line or organoid transplantation models generally produce tumors in ectopic locations,...

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Bibliografske podrobnosti
izdano v:Nat Protoc
Main Authors: Roper, Jatin, Tammela, Tuomas, Akkad, Adam, Almeqdadi, Mohammad, Santos, Sebastian B., Jacks, Tyler, Yilmaz, Ömer H.
Format: Artigo
Jezik:Inglês
Izdano: 2018
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC6145089/
https://ncbi.nlm.nih.gov/pubmed/29300388
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2017.136
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