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Continuous directed evolution of proteins with improved soluble expression
We report the development of soluble expression phage-assisted continuous evolution (SE-PACE), a system for rapidly evolving proteins with increased soluble expression. Through use of a PACE-compatible AND gate that uses a split-intein pIII, SE-PACE enables two simultaneous positive selections to ev...
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| Publicado no: | Nat Chem Biol |
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| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6143403/ https://ncbi.nlm.nih.gov/pubmed/30127387 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41589-018-0121-5 |
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