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Quantitation of Intact Proteins in Human Plasma Using Top-down Parallel Reaction Monitoring-MS
Direct quantitation of proteins in complex biological matrices by mass spectrometry remains a challenge. Here we describe a novel top-down parallel reaction monitoring-mass spectrometry (PRM-MS) assay, enabled by microflow LC-nanospray MS using a silicon microfluidic LC-MS chip. We demonstrated dire...
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| Vydáno v: | Anal Chem |
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| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2018
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6143396/ https://ncbi.nlm.nih.gov/pubmed/30192519 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.8b02699 |
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