Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels

Post-transcriptional chemical modifications of (t)RNA molecules are crucial in fundamental biological processes, such as translation. Despite their biological importance and accumulating evidence linking them to various human diseases, technical challenges have limited their detection and accurate q...

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Veröffentlicht in:RNA
Hauptverfasser: Sarin, L. Peter, Kienast, Sandra D., Leufken, Johannes, Ross, Robert L., Dziergowska, Agnieszka, Debiec, Katarzyna, Sochacka, Elzbieta, Limbach, Patrick A., Fufezan, Christian, Drexler, Hannes C.A., Leidel, Sebastian A.
Format: Artigo
Sprache:Inglês
Veröffentlicht: Cold Spring Harbor Laboratory Press 2018
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC6140458/
https://ncbi.nlm.nih.gov/pubmed/30012570
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.065482.117
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