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Investigation of Detection Limits and the Influence of DNA Extraction and Primer Choice on the Observed Microbial Communities in Drinking Water Samples Using 16S rRNA Gene Amplicon Sequencing

In recent years, 16S rRNA gene amplicon sequencing has been widely adopted for analyzing the microbial communities in drinking water (DW). However, no comprehensive attempts have been made to illuminate the inherent method biases specifically relating to DW communities. In this study, we investigate...

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Publié dans:Front Microbiol
Auteurs principaux: Brandt, Jakob, Albertsen, Mads
Format: Artigo
Langue:Inglês
Publié: Frontiers Media S.A. 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6137089/
https://ncbi.nlm.nih.gov/pubmed/30245681
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2018.02140
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