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Super-Resolution Single Molecule FISH at the Drosophila Neuromuscular Junction

The lack of an effective, simple, and highly sensitive protocol for fluorescent in situ hybridization (FISH) at the Drosophila larval neuromuscular junction (NMJ) has hampered the study of mRNA biology. Here, we describe our modified single molecule FISH (smFISH) methods that work well in whole moun...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Titlow, Joshua S., Yang, Lu, Parton, Richard M., Palanca, Ana, Davis, Ilan
Formato: Artigo
Idioma:Inglês
Publicado em: Springer New York 2017
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6128253/
https://ncbi.nlm.nih.gov/pubmed/29130196
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-7213-5_10
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