Single molecule fluorescence in situ hybridisation for quantitating post-transcriptional regulation in Drosophila brains

RNA in situ hybridization is a powerful method to investigate post-transcriptional regulation, but analysis of intracellular mRNA distributions in thick, complex tissues like the brain poses significant challenges. Here, we describe the application of single-molecule fluorescent in situ hybridizatio...

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Detalhes bibliográficos
Publicado no:Methods
Main Authors: Yang, Lu, Titlow, Josh, Ennis, Darragh, Smith, Carlas, Mitchell, Jessica, Young, Florence L., Waddell, Scott, Ish-Horowicz, David, Davis, Ilan
Formato: Artigo
Idioma:Inglês
Publicado em: Academic Press 2017
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5595163/
https://ncbi.nlm.nih.gov/pubmed/28651965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2017.06.025
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