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A tRNA-based multiplex sgRNA expression system in zebrafish and its application to generation of transgenic albino fish
The CRISPR/Cas9 system can be introduced into zebrafish as transgenes. Namely, expression of single-guide RNA (sgRNA) and controlled expression of Cas9 in transgenic zebrafish enables the study of gene functions in specific cell types. This transgenic CRISPR/Cas9 approach would be more useful if mul...
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| 出版年: | Sci Rep |
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| 主要な著者: | , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group UK
2018
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6127137/ https://ncbi.nlm.nih.gov/pubmed/30190522 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-018-31476-5 |
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