A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation

This protocol describes a novel and easy net mold-based method to create three-dimensional (3-D) cardiac tissues without additional scaffold material. Human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), human cardiac fibroblasts (HCFs), and human umbilical vein endothelial cells (...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Bai, Yang, Yeung, Enoch, Lui, Cecillia, Ong, Chin Siang, Pitaktong, Isaree, Huang, Chenyu, Inoue, Takahiro, Matsushita, Hiroshi, Ma, Chunye, Hibino, Narutoshi
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2018
Subjects:
Bioengineering
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC6126624/
https://ncbi.nlm.nih.gov/pubmed/30124650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58252
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