Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates....

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Dades bibliogràfiques
Publicat a:J Vis Exp
Autors principals: Ong, Chin Siang, Fukunishi, Takuma, Nashed, Andrew, Blazeski, Adriana, Zhang, Huaitao, Hardy, Samantha, DiSilvestre, Deborah, Vricella, Luca, Conte, John, Tung, Leslie, Tomaselli, Gordon, Hibino, Narutoshi
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2017
Matèries:
Bioengineering
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608529/
https://ncbi.nlm.nih.gov/pubmed/28715377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55438
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