Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates....

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Ong, Chin Siang, Fukunishi, Takuma, Nashed, Andrew, Blazeski, Adriana, Zhang, Huaitao, Hardy, Samantha, DiSilvestre, Deborah, Vricella, Luca, Conte, John, Tung, Leslie, Tomaselli, Gordon, Hibino, Narutoshi
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2017
Assuntos:
Bioengineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608529/
https://ncbi.nlm.nih.gov/pubmed/28715377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55438
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