Creation of Cardiac Tissue Exhibiting Mechanical Integration of Spheroids Using 3D Bioprinting

This protocol describes 3D bioprinting of cardiac tissue without the use of biomaterials, using only cells. Cardiomyocytes, endothelial cells and fibroblasts are first isolated, counted and mixed at desired cell ratios. They are co-cultured in individual wells in ultra-low attachment 96-well plates....

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Podrobná bibliografie
Vydáno v:J Vis Exp
Hlavní autoři: Ong, Chin Siang, Fukunishi, Takuma, Nashed, Andrew, Blazeski, Adriana, Zhang, Huaitao, Hardy, Samantha, DiSilvestre, Deborah, Vricella, Luca, Conte, John, Tung, Leslie, Tomaselli, Gordon, Hibino, Narutoshi
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2017
Témata:
Bioengineering
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5608529/
https://ncbi.nlm.nih.gov/pubmed/28715377
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/55438
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