Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Number and brightness is a calibration-free fluorescence fluctuation spectroscopy (FFS) technique for detecting protein homo-oligomerization. It can be employed using a conventional confocal microscope equipped with digital detectors. A protocol for the use of the technique in vitro is shown by mean...

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Publicat a:J Vis Exp
Autors principals: Nolan, Rory, Alvarez, Luis A., Griffiths, Samuel C., Elegheert, Jonathan, Siebold, Christian, Padilla-Parra, Sergi
Format: Artigo
Idioma:Inglês
Publicat: MyJove Corporation 2018
Matèries:
This Month in JoVE
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6126508/
https://ncbi.nlm.nih.gov/pubmed/30080195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/58157
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