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Preparation of Three-Dimensional (3D) Human Liver (HepaRG) Cultures for Histochemical and Immunohistochemical Staining and Light Microscopic Evaluation

The use of three-dimensional (3D) in vitro culture systems (spheroids; organoids) in biomolecular and drug discovery research has become increasingly popular. The popularity is due, in part, to a diminished reliance on animal bioassays and a desire to develop physiologically relevant cell culture sy...

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Publicat a:Toxicol Pathol
Autors principals: Clayton, Natasha P., Burwell, Alanna, Jensen, Heather, Williams, Barbara F., Brown, Quashana D., Ovwigho, Pamela, Ramaiahgari, Sreenivasa, Hermon, Tonia, Dixon, Darlene
Format: Artigo
Idioma:Inglês
Publicat: 2018
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC6117202/
https://ncbi.nlm.nih.gov/pubmed/30089414
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1177/0192623318789069
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