Development and Optimization of Metagenomic Next-Generation Sequencing Methods for Cerebrospinal Fluid Diagnostics

The purpose of this study was to develop and optimize different processing, extraction, amplification, and sequencing methods for metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) specimens. We applied mNGS to 10 CSF samples with known standard-of-care testing (SoC) results...

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Detalhes bibliográficos
Publicado no:J Clin Microbiol
Main Authors: Simner, Patricia J., Miller, Heather B., Breitwieser, Florian P., Pinilla Monsalve, Gabriel, Pardo, Carlos A., Salzberg, Steven L., Sears, Cynthia L., Thomas, David L., Eberhart, Charles G., Carroll, Karen C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2018
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6113476/
https://ncbi.nlm.nih.gov/pubmed/29976594
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00472-18
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