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Determination of Plasma Membrane Partitioning for Peripherally-associated Proteins

This method provides a fast approach for the determination of plasma membrane partitioning of any fluorescently-tagged peripherally-associated protein using the profiles of fluorescence intensity across the plasma membrane. Measured fluorescence profiles are fitted by a model for membrane and cytopl...

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Détails bibliographiques
Publié dans:J Vis Exp
Auteurs principaux: Vosolsobě, Stanislav, Schwarzerová, Kateřina, Petrášek, Jan
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2018
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC6101750/
https://ncbi.nlm.nih.gov/pubmed/29985355
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57837
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