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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
MicroRNAs (miRNAs) are a class of small noncoding RNAs that post-transcriptionally regulate cellular gene expression. MiRNAs bind to the 3' untranslated region (UTR) of target mRNA to inhibit protein translation or in some instances cause mRNA degradation. The binding of the miRNA to the 3'...
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| Publicado no: | J Vis Exp |
|---|---|
| Main Authors: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2018
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6101703/ https://ncbi.nlm.nih.gov/pubmed/29985341 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57786 |
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